Vegfa Stable Expression Cell Line (4T1)

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  • 细胞图0 Vegfa Stable Expression Cell Line (4T1)-细胞图0
  • 细胞图1 Vegfa Stable Expression Cell Line (4T1)-细胞图1
  • 质粒图0 Vegfa Stable Expression Cell Line (4T1)-质粒图0
  • Vegfa Stable Expression Cell Line (4T1) Vegfa Stable Expression Cell Line (4T1)-产品图
细胞图0 Vegfa Stable Expression Cell Line (4T1)-细胞图0
细胞图1 Vegfa Stable Expression Cell Line (4T1)-细胞图1
质粒图0 Vegfa Stable Expression Cell Line (4T1)-质粒图0
Vegfa Stable Expression Cell Line (4T1) Vegfa Stable Expression Cell Line (4T1)-产品图
Vegfa Stable Expression Cell Line (4T1) Copy Product Info
Catalog#： YOE-L016
Size： 1*10^6
Culture method： 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
STR Report
Datasheet (Use Instruction)
  • 细胞图0 Vegfa Stable Expression Cell Line (4T1)-细胞图0
  • 细胞图1 Vegfa Stable Expression Cell Line (4T1)-细胞图1
  • 质粒图0 Vegfa Stable Expression Cell Line (4T1)-质粒图0
  • Vegfa Stable Expression Cell Line (4T1) Vegfa Stable Expression Cell Line (4T1)-产品图
Vegfa Stable Expression Cell Line (4T1) Copy Product Info
Catalog#： YOE-L016
Size： 1*10^6
Culture method： 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L016 Transcript NM_001025250.3
Cell name Vegfa Stable Expression Cell Line (4T1) Species Mouse
Gene Name Vegfa Passage ratio 1:2~1:4
Cell Morphology Epithelial-like, adherent Antibiotic Concentration Puro=3μg/ml
Culture method 88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate Mycoplasma test negative
Catalog
YOE-L016
Cell name
Vegfa Stable Expression Cell Line (4T1)
Gene Name
Vegfa
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Transcript
NM_001025250.3
Species
Mouse
Antibiotic Concentration
Puro=3μg/ml
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate

Product Description

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
Gene Expression Validation
No. Sample mVegfaCтMean Q-mGAPDHCтMean ΔCтMean ΔΔCт Expression Rate
1 Vegfa Stable Expression Cell Line (4T1) 23.633 12.827 10.806 -2.977 7.873
2 4T1_Ctrl(Puro) 27.698 13.915 13.783 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:4T1		 STR Info (Cell bank)
Cell Line:4T1
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.019.018.019.0
4-2 21.321.3
6-7 12.012.0
19-2 13.013.0
1-2 17.017.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.015.016.0
3-2 14.015.014.015.0
2-1 16.017.016.017.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 18.020.018.019.020.0
17-2 15.015.0
12-1 16.016.0
5-5 14.014.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
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    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
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    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Citations:
Product Catalog#：YOE-L016
Product Name：Vegfa Stable Expression Cell Line (4T1)
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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